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mta1 crispr activation plasmid  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology mta1 crispr activation plasmid
    Figure 1. Leptin upregulates <t>MTA1</t> expression in human breast cancer cells. MTA1 mRNA expression was analyzed using qPCR in MDA-MB-231 (A) and Hs 578T cells (B) after leptin treatment for 24 h. Results are expressed as mean ± SD; * p < 0.05 and ** p < 0.01 vs. untreated control. MTA1 protein expression was assessed using Western blot in MDA-MB-231 (C) and Hs 578T cells (D) after 24 h of leptin treatment.
    Mta1 Crispr Activation Plasmid, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mta1+crispr+activation+plasmid/MTA1+CRISPR+Activation+Plasmid/10__3390_slash_ijms26125726-99-30-34
    Average 93 stars, based on 1 article reviews
    mta1 crispr activation plasmid - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Leptin-Upregulated Metastasis-Associated Protein 1 Promotes Vasculogenic Mimicry in Breast Cancer Cells"

    Article Title: Leptin-Upregulated Metastasis-Associated Protein 1 Promotes Vasculogenic Mimicry in Breast Cancer Cells

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms26125726

    Figure 1. Leptin upregulates MTA1 expression in human breast cancer cells. MTA1 mRNA expression was analyzed using qPCR in MDA-MB-231 (A) and Hs 578T cells (B) after leptin treatment for 24 h. Results are expressed as mean ± SD; * p < 0.05 and ** p < 0.01 vs. untreated control. MTA1 protein expression was assessed using Western blot in MDA-MB-231 (C) and Hs 578T cells (D) after 24 h of leptin treatment.
    Figure Legend Snippet: Figure 1. Leptin upregulates MTA1 expression in human breast cancer cells. MTA1 mRNA expression was analyzed using qPCR in MDA-MB-231 (A) and Hs 578T cells (B) after leptin treatment for 24 h. Results are expressed as mean ± SD; * p < 0.05 and ** p < 0.01 vs. untreated control. MTA1 protein expression was assessed using Western blot in MDA-MB-231 (C) and Hs 578T cells (D) after 24 h of leptin treatment.

    Techniques Used: Expressing, Control, Western Blot

    Figure 2. Leptin upregulates MTA1 expression via the Ob-R/STAT3 pathway in human breast cancer cells. MTA1 protein expression was evaluated using Western blot in MDA-MB-231 (A,C) and Hs 578T cells (B,D) after 24 h of leptin treatment in the presence or absence of Ob-R BP (A,B) or AG490 (C,D).
    Figure Legend Snippet: Figure 2. Leptin upregulates MTA1 expression via the Ob-R/STAT3 pathway in human breast cancer cells. MTA1 protein expression was evaluated using Western blot in MDA-MB-231 (A,C) and Hs 578T cells (B,D) after 24 h of leptin treatment in the presence or absence of Ob-R BP (A,B) or AG490 (C,D).

    Techniques Used: Expressing, Western Blot

    Figure 3. MTA1 overexpression promotes VM in human breast cancer cells. MDA-MB-231 (A,C,E) and Hs 578T cells (B,D,F) were transfected with the MTA1 CRISPR activation plasmid for 48 h. MTA1 protein expression was assessed using Western blot in MDA-MB-231 (A) and Hs 578T cells (B). VM was performed using a 3D culture assay for 16 h in MDA-MB-231 (C) and Hs 578T cells (D) (40× mag- nification; scale bar = 500 µm). Results are expressed as mean ± SD; *** p < 0.001 vs. control plasmid. Expression of VM-related proteins was evaluated using Western blot in MDA-MB-231 (E) and Hs 578T cells (F).
    Figure Legend Snippet: Figure 3. MTA1 overexpression promotes VM in human breast cancer cells. MDA-MB-231 (A,C,E) and Hs 578T cells (B,D,F) were transfected with the MTA1 CRISPR activation plasmid for 48 h. MTA1 protein expression was assessed using Western blot in MDA-MB-231 (A) and Hs 578T cells (B). VM was performed using a 3D culture assay for 16 h in MDA-MB-231 (C) and Hs 578T cells (D) (40× mag- nification; scale bar = 500 µm). Results are expressed as mean ± SD; *** p < 0.001 vs. control plasmid. Expression of VM-related proteins was evaluated using Western blot in MDA-MB-231 (E) and Hs 578T cells (F).

    Techniques Used: Over Expression, Transfection, CRISPR, Activation Assay, Plasmid Preparation, Expressing, Western Blot, Control

    Figure 4. MTA1 silencing inhibits leptin-induced VM in human breast cancer cells. MDA-MB- 231 (A,C,E) and Hs 578T cells (B,D,F) were treated with leptin 48 h after transfection with MTA1 siRNA. MTA1 protein expression was evaluated using Western blot in MDA-MB-231 (A) and Hs 578T cells (B). VM was performed using a 3D culture assay for 16 h in MDA-MB-231 (C) and Hs 578T cells (D) (40× magnification; scale bar = 250 µm). Results are expressed as mean ± SD; *** p < 0.001 vs. untreated control; ### p < 0.001 vs. control siRNA. Expression of VM-related proteins was evaluated using Western blot in MDA-MB-231 (E) and Hs 578T cells (F).
    Figure Legend Snippet: Figure 4. MTA1 silencing inhibits leptin-induced VM in human breast cancer cells. MDA-MB- 231 (A,C,E) and Hs 578T cells (B,D,F) were treated with leptin 48 h after transfection with MTA1 siRNA. MTA1 protein expression was evaluated using Western blot in MDA-MB-231 (A) and Hs 578T cells (B). VM was performed using a 3D culture assay for 16 h in MDA-MB-231 (C) and Hs 578T cells (D) (40× magnification; scale bar = 250 µm). Results are expressed as mean ± SD; *** p < 0.001 vs. untreated control; ### p < 0.001 vs. control siRNA. Expression of VM-related proteins was evaluated using Western blot in MDA-MB-231 (E) and Hs 578T cells (F).

    Techniques Used: Transfection, Expressing, Western Blot, Control

    Related Articles

    Multiple Displacement Amplification:

    Article Title: Leptin-Upregulated Metastasis-Associated Protein 1 Promotes Vasculogenic Mimicry in Breast Cancer Cells
    Article Snippet: .. MDA-MB-231 cells (1.5 × 10 5 ) and Hs 578T cells (2.0 × 10 5 ) were cultured under optimal conditions and transfected with 1 μg of the control or 0.5 μg of the MTA1 CRISPR activation plasmid (Santa Cruz, Danvers, MA, USA) for 48 h. Transfection was performed using the UltraCruz transfection reagent (Santa Cruz), according to the manufacturer’s protocol. ..

    Article Title: Leptin-Upregulated Metastasis-Associated Protein 1 Promotes Vasculogenic Mimicry in Breast Cancer Cells
    Article Snippet: .. MDA-MB-231 cells (1.5 × 105) and Hs 578T cells (2.0 × 105) were cultured under optimal conditions and transfected with 1 μg of the control or 0.5 μg of the MTA1 CRISPR activation plasmid (Santa Cruz, Danvers, MA, USA) for 48 h. Transfection was performed using the UltraCruz transfection reagent (Santa Cruz), according to the manufacturer’s protocol. ..

    Cell Culture:

    Article Title: Leptin-Upregulated Metastasis-Associated Protein 1 Promotes Vasculogenic Mimicry in Breast Cancer Cells
    Article Snippet: .. MDA-MB-231 cells (1.5 × 10 5 ) and Hs 578T cells (2.0 × 10 5 ) were cultured under optimal conditions and transfected with 1 μg of the control or 0.5 μg of the MTA1 CRISPR activation plasmid (Santa Cruz, Danvers, MA, USA) for 48 h. Transfection was performed using the UltraCruz transfection reagent (Santa Cruz), according to the manufacturer’s protocol. ..

    Article Title: Leptin-Upregulated Metastasis-Associated Protein 1 Promotes Vasculogenic Mimicry in Breast Cancer Cells
    Article Snippet: .. MDA-MB-231 cells (1.5 × 105) and Hs 578T cells (2.0 × 105) were cultured under optimal conditions and transfected with 1 μg of the control or 0.5 μg of the MTA1 CRISPR activation plasmid (Santa Cruz, Danvers, MA, USA) for 48 h. Transfection was performed using the UltraCruz transfection reagent (Santa Cruz), according to the manufacturer’s protocol. ..

    Transfection:

    Article Title: Leptin-Upregulated Metastasis-Associated Protein 1 Promotes Vasculogenic Mimicry in Breast Cancer Cells
    Article Snippet: .. MDA-MB-231 cells (1.5 × 10 5 ) and Hs 578T cells (2.0 × 10 5 ) were cultured under optimal conditions and transfected with 1 μg of the control or 0.5 μg of the MTA1 CRISPR activation plasmid (Santa Cruz, Danvers, MA, USA) for 48 h. Transfection was performed using the UltraCruz transfection reagent (Santa Cruz), according to the manufacturer’s protocol. ..

    Article Title: Leptin-Upregulated Metastasis-Associated Protein 1 Promotes Vasculogenic Mimicry in Breast Cancer Cells
    Article Snippet: .. MDA-MB-231 cells (1.5 × 105) and Hs 578T cells (2.0 × 105) were cultured under optimal conditions and transfected with 1 μg of the control or 0.5 μg of the MTA1 CRISPR activation plasmid (Santa Cruz, Danvers, MA, USA) for 48 h. Transfection was performed using the UltraCruz transfection reagent (Santa Cruz), according to the manufacturer’s protocol. ..

    Control:

    Article Title: Leptin-Upregulated Metastasis-Associated Protein 1 Promotes Vasculogenic Mimicry in Breast Cancer Cells
    Article Snippet: .. MDA-MB-231 cells (1.5 × 10 5 ) and Hs 578T cells (2.0 × 10 5 ) were cultured under optimal conditions and transfected with 1 μg of the control or 0.5 μg of the MTA1 CRISPR activation plasmid (Santa Cruz, Danvers, MA, USA) for 48 h. Transfection was performed using the UltraCruz transfection reagent (Santa Cruz), according to the manufacturer’s protocol. ..

    Article Title: Leptin-Upregulated Metastasis-Associated Protein 1 Promotes Vasculogenic Mimicry in Breast Cancer Cells
    Article Snippet: .. MDA-MB-231 cells (1.5 × 105) and Hs 578T cells (2.0 × 105) were cultured under optimal conditions and transfected with 1 μg of the control or 0.5 μg of the MTA1 CRISPR activation plasmid (Santa Cruz, Danvers, MA, USA) for 48 h. Transfection was performed using the UltraCruz transfection reagent (Santa Cruz), according to the manufacturer’s protocol. ..

    CRISPR:

    Article Title: Leptin-Upregulated Metastasis-Associated Protein 1 Promotes Vasculogenic Mimicry in Breast Cancer Cells
    Article Snippet: .. MDA-MB-231 cells (1.5 × 10 5 ) and Hs 578T cells (2.0 × 10 5 ) were cultured under optimal conditions and transfected with 1 μg of the control or 0.5 μg of the MTA1 CRISPR activation plasmid (Santa Cruz, Danvers, MA, USA) for 48 h. Transfection was performed using the UltraCruz transfection reagent (Santa Cruz), according to the manufacturer’s protocol. ..

    Article Title: Leptin-Upregulated Metastasis-Associated Protein 1 Promotes Vasculogenic Mimicry in Breast Cancer Cells
    Article Snippet: .. MDA-MB-231 cells (1.5 × 105) and Hs 578T cells (2.0 × 105) were cultured under optimal conditions and transfected with 1 μg of the control or 0.5 μg of the MTA1 CRISPR activation plasmid (Santa Cruz, Danvers, MA, USA) for 48 h. Transfection was performed using the UltraCruz transfection reagent (Santa Cruz), according to the manufacturer’s protocol. ..

    Activation Assay:

    Article Title: Leptin-Upregulated Metastasis-Associated Protein 1 Promotes Vasculogenic Mimicry in Breast Cancer Cells
    Article Snippet: .. MDA-MB-231 cells (1.5 × 10 5 ) and Hs 578T cells (2.0 × 10 5 ) were cultured under optimal conditions and transfected with 1 μg of the control or 0.5 μg of the MTA1 CRISPR activation plasmid (Santa Cruz, Danvers, MA, USA) for 48 h. Transfection was performed using the UltraCruz transfection reagent (Santa Cruz), according to the manufacturer’s protocol. ..

    Article Title: Leptin-Upregulated Metastasis-Associated Protein 1 Promotes Vasculogenic Mimicry in Breast Cancer Cells
    Article Snippet: .. MDA-MB-231 cells (1.5 × 105) and Hs 578T cells (2.0 × 105) were cultured under optimal conditions and transfected with 1 μg of the control or 0.5 μg of the MTA1 CRISPR activation plasmid (Santa Cruz, Danvers, MA, USA) for 48 h. Transfection was performed using the UltraCruz transfection reagent (Santa Cruz), according to the manufacturer’s protocol. ..

    Plasmid Preparation:

    Article Title: Leptin-Upregulated Metastasis-Associated Protein 1 Promotes Vasculogenic Mimicry in Breast Cancer Cells
    Article Snippet: .. MDA-MB-231 cells (1.5 × 10 5 ) and Hs 578T cells (2.0 × 10 5 ) were cultured under optimal conditions and transfected with 1 μg of the control or 0.5 μg of the MTA1 CRISPR activation plasmid (Santa Cruz, Danvers, MA, USA) for 48 h. Transfection was performed using the UltraCruz transfection reagent (Santa Cruz), according to the manufacturer’s protocol. ..

    Article Title: Leptin-Upregulated Metastasis-Associated Protein 1 Promotes Vasculogenic Mimicry in Breast Cancer Cells
    Article Snippet: .. MDA-MB-231 cells (1.5 × 105) and Hs 578T cells (2.0 × 105) were cultured under optimal conditions and transfected with 1 μg of the control or 0.5 μg of the MTA1 CRISPR activation plasmid (Santa Cruz, Danvers, MA, USA) for 48 h. Transfection was performed using the UltraCruz transfection reagent (Santa Cruz), according to the manufacturer’s protocol. ..



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    mta1 crispr activation plasmid  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology mta1 crispr activation plasmid
    Figure 1. Leptin upregulates <t>MTA1</t> expression in human breast cancer cells. MTA1 mRNA expression was analyzed using qPCR in MDA-MB-231 (A) and Hs 578T cells (B) after leptin treatment for 24 h. Results are expressed as mean ± SD; * p < 0.05 and ** p < 0.01 vs. untreated control. MTA1 protein expression was assessed using Western blot in MDA-MB-231 (C) and Hs 578T cells (D) after 24 h of leptin treatment.
    Mta1 Crispr Activation Plasmid, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mta1+crispr+activation+plasmid/MTA1+CRISPR+Activation+Plasmid/10__3390_slash_ijms26125726-99-30-34
    Average 93 stars, based on 1 article reviews
    mta1 crispr activation plasmid - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 1. Leptin upregulates MTA1 expression in human breast cancer cells. MTA1 mRNA expression was analyzed using qPCR in MDA-MB-231 (A) and Hs 578T cells (B) after leptin treatment for 24 h. Results are expressed as mean ± SD; * p < 0.05 and ** p < 0.01 vs. untreated control. MTA1 protein expression was assessed using Western blot in MDA-MB-231 (C) and Hs 578T cells (D) after 24 h of leptin treatment.

    Journal: International Journal of Molecular Sciences

    Article Title: Leptin-Upregulated Metastasis-Associated Protein 1 Promotes Vasculogenic Mimicry in Breast Cancer Cells

    doi: 10.3390/ijms26125726

    Figure Lengend Snippet: Figure 1. Leptin upregulates MTA1 expression in human breast cancer cells. MTA1 mRNA expression was analyzed using qPCR in MDA-MB-231 (A) and Hs 578T cells (B) after leptin treatment for 24 h. Results are expressed as mean ± SD; * p < 0.05 and ** p < 0.01 vs. untreated control. MTA1 protein expression was assessed using Western blot in MDA-MB-231 (C) and Hs 578T cells (D) after 24 h of leptin treatment.

    Article Snippet: MDA-MB-231 cells (1.5 × 105) and Hs 578T cells (2.0 × 105) were cultured under optimal conditions and transfected with 1 μg of the control or 0.5 μg of the MTA1 CRISPR activation plasmid (Santa Cruz, Danvers, MA, USA) for 48 h. Transfection was performed using the UltraCruz transfection reagent (Santa Cruz), according to the manufacturer’s protocol.

    Techniques: Expressing, Control, Western Blot

    Figure 2. Leptin upregulates MTA1 expression via the Ob-R/STAT3 pathway in human breast cancer cells. MTA1 protein expression was evaluated using Western blot in MDA-MB-231 (A,C) and Hs 578T cells (B,D) after 24 h of leptin treatment in the presence or absence of Ob-R BP (A,B) or AG490 (C,D).

    Journal: International Journal of Molecular Sciences

    Article Title: Leptin-Upregulated Metastasis-Associated Protein 1 Promotes Vasculogenic Mimicry in Breast Cancer Cells

    doi: 10.3390/ijms26125726

    Figure Lengend Snippet: Figure 2. Leptin upregulates MTA1 expression via the Ob-R/STAT3 pathway in human breast cancer cells. MTA1 protein expression was evaluated using Western blot in MDA-MB-231 (A,C) and Hs 578T cells (B,D) after 24 h of leptin treatment in the presence or absence of Ob-R BP (A,B) or AG490 (C,D).

    Article Snippet: MDA-MB-231 cells (1.5 × 105) and Hs 578T cells (2.0 × 105) were cultured under optimal conditions and transfected with 1 μg of the control or 0.5 μg of the MTA1 CRISPR activation plasmid (Santa Cruz, Danvers, MA, USA) for 48 h. Transfection was performed using the UltraCruz transfection reagent (Santa Cruz), according to the manufacturer’s protocol.

    Techniques: Expressing, Western Blot

    Figure 3. MTA1 overexpression promotes VM in human breast cancer cells. MDA-MB-231 (A,C,E) and Hs 578T cells (B,D,F) were transfected with the MTA1 CRISPR activation plasmid for 48 h. MTA1 protein expression was assessed using Western blot in MDA-MB-231 (A) and Hs 578T cells (B). VM was performed using a 3D culture assay for 16 h in MDA-MB-231 (C) and Hs 578T cells (D) (40× mag- nification; scale bar = 500 µm). Results are expressed as mean ± SD; *** p < 0.001 vs. control plasmid. Expression of VM-related proteins was evaluated using Western blot in MDA-MB-231 (E) and Hs 578T cells (F).

    Journal: International Journal of Molecular Sciences

    Article Title: Leptin-Upregulated Metastasis-Associated Protein 1 Promotes Vasculogenic Mimicry in Breast Cancer Cells

    doi: 10.3390/ijms26125726

    Figure Lengend Snippet: Figure 3. MTA1 overexpression promotes VM in human breast cancer cells. MDA-MB-231 (A,C,E) and Hs 578T cells (B,D,F) were transfected with the MTA1 CRISPR activation plasmid for 48 h. MTA1 protein expression was assessed using Western blot in MDA-MB-231 (A) and Hs 578T cells (B). VM was performed using a 3D culture assay for 16 h in MDA-MB-231 (C) and Hs 578T cells (D) (40× mag- nification; scale bar = 500 µm). Results are expressed as mean ± SD; *** p < 0.001 vs. control plasmid. Expression of VM-related proteins was evaluated using Western blot in MDA-MB-231 (E) and Hs 578T cells (F).

    Article Snippet: MDA-MB-231 cells (1.5 × 105) and Hs 578T cells (2.0 × 105) were cultured under optimal conditions and transfected with 1 μg of the control or 0.5 μg of the MTA1 CRISPR activation plasmid (Santa Cruz, Danvers, MA, USA) for 48 h. Transfection was performed using the UltraCruz transfection reagent (Santa Cruz), according to the manufacturer’s protocol.

    Techniques: Over Expression, Transfection, CRISPR, Activation Assay, Plasmid Preparation, Expressing, Western Blot, Control

    Figure 4. MTA1 silencing inhibits leptin-induced VM in human breast cancer cells. MDA-MB- 231 (A,C,E) and Hs 578T cells (B,D,F) were treated with leptin 48 h after transfection with MTA1 siRNA. MTA1 protein expression was evaluated using Western blot in MDA-MB-231 (A) and Hs 578T cells (B). VM was performed using a 3D culture assay for 16 h in MDA-MB-231 (C) and Hs 578T cells (D) (40× magnification; scale bar = 250 µm). Results are expressed as mean ± SD; *** p < 0.001 vs. untreated control; ### p < 0.001 vs. control siRNA. Expression of VM-related proteins was evaluated using Western blot in MDA-MB-231 (E) and Hs 578T cells (F).

    Journal: International Journal of Molecular Sciences

    Article Title: Leptin-Upregulated Metastasis-Associated Protein 1 Promotes Vasculogenic Mimicry in Breast Cancer Cells

    doi: 10.3390/ijms26125726

    Figure Lengend Snippet: Figure 4. MTA1 silencing inhibits leptin-induced VM in human breast cancer cells. MDA-MB- 231 (A,C,E) and Hs 578T cells (B,D,F) were treated with leptin 48 h after transfection with MTA1 siRNA. MTA1 protein expression was evaluated using Western blot in MDA-MB-231 (A) and Hs 578T cells (B). VM was performed using a 3D culture assay for 16 h in MDA-MB-231 (C) and Hs 578T cells (D) (40× magnification; scale bar = 250 µm). Results are expressed as mean ± SD; *** p < 0.001 vs. untreated control; ### p < 0.001 vs. control siRNA. Expression of VM-related proteins was evaluated using Western blot in MDA-MB-231 (E) and Hs 578T cells (F).

    Article Snippet: MDA-MB-231 cells (1.5 × 105) and Hs 578T cells (2.0 × 105) were cultured under optimal conditions and transfected with 1 μg of the control or 0.5 μg of the MTA1 CRISPR activation plasmid (Santa Cruz, Danvers, MA, USA) for 48 h. Transfection was performed using the UltraCruz transfection reagent (Santa Cruz), according to the manufacturer’s protocol.

    Techniques: Transfection, Expressing, Western Blot, Control